While Steve Clarke has his pick of the squad who qualified for the Euros, Jaroslav Silhavy is missing one star in Lukáš Provod.
The Slavia Praha star was ruled out of the tournament with a knee injury but aside from the midfielder Silhavy has an array of players to choose from.
Among them Alex Kral and Tomas Soucek who are set to feature in the group match.
And Scotland have Che Adams and Kieran Tierney in amongst the squad who will play in their first major tournament in 23 years.
Here is everything you need to know about the match.
When is the match?
The game will kick-off at 2pm on Monday, June 14.
Where can I watch?
The match will be shown on BBC One and on the BBC iPlayer with coverage beginning at 1.15pm.
Scotland: Marshall; O'Donnell, Hendry, Hanley, Cooper, Robertson; McTominay, McGinn, Armstrong; Dykes, Christie
Czech Republic: Vaclik; Coufal, Celustka, Kalas, Boril; Soucek, Kral; Masopust, Darida, Jankto; Schick
Scotland - 39/20
Draw - 19/20
Czech Republic - 33/20
A home crowd for Scotland in Hampden Park, Glasgow will spur them onto a slim victory which will follow on from their good form in their warm-ups. Scotland 1-0 Czech Republic.