Jobs are being shed at the Royal Children's Hospital in Brisbane as part of a bid to save taxpayers $15 million.
Children's Health Queensland Hospital and Health Service has confirmed it is cutting 99 full-time jobs, or five per cent of the workforce.
This will see positions reduced at the Royal Children's Hospital, the Child and Youth Community Health Service and the Child and Youth Mental Health Service.
Chair of the government agency, Susan Johnston, said redundancies were likely, but would not detail exactly where the job cuts would occur.
"Natural attrition of the workforce and the removal of current non-frontline roles will be a priority," she said in a statement on Monday.
"However, it is likely that some redundancies will be required.
"Every part of the organisation ... will be affected by these budget reductions and every aspect of our service delivery will be reviewed to identify options and strategies."
Opposition Leader Annastacia Palaszczuk said Health Minister Lawrence Springborg needed to detail what frontline jobs and services were being cut.
"These latest cuts to jobs and services are Mr Springborg's Christmas present to parents across Queensland," she said.
"He is the minister and he cannot escape responsibility for these cuts."
In September, Mr Springborg announced 2754 jobs would be shed at Queensland Health, including 1537 full-time positions at Hospital and Health Services, under a budget plan to slash 14,000 public service positions.
The minister's office declined to comment directly on Monday.
Queensland Nurses Union officials were given a government briefing.